What is the minimum sample amount required when performing vial sampling with the First Defender RMX?

• A. 0.5 mL
• B. 1 mL
• C. 2 mL
• D. 5 mL

Answer: (B)

The minimum amount needed is 1 mL. This volume provides enough liquid to fill the vial’s sampling path and let the device properly aspirate and meter the sample for accurate analysis. Using less than this can leave air gaps or not deliver enough analyte to the reaction or detection chamber, which can lead to unreliable or failed results. Volumes larger than 1 mL aren’t required for the procedure and don’t improve accuracy; they simply use more sample than necessary.